16S of human: Healthy-Control Subject 24 - SRA

SRX25244816: 16S of human: Healthy-Control Subject 24
1 ILLUMINA (Illumina MiSeq) run: 61,360 spots, 30.8M bases, 16.4Mb downloads

Design: A stool sample was collected from each participant at the time of the enrollment by spontaneous or enema defecation using a self-collection stool kit (CeGat GmbH, Tbingen, Baden-Wrttemberg, Germany). The sampling kit contained a liquid-based DNA preservation solution and samples were stored at room temperature (20-25 C) until DNA extraction. Total DNA was extracted using the Zymo BiomicsDNA/Rna Miniprep Kit (Zymo Research) and quality was checked with Qubit dsDNA BR or HS (Thermo Fisher), as per manufacturers protocol. Twenty ng of DNA were used for library preparation by amplifying the V3-V4 hypervariable regions of the bacterial 16S rRNA gene using the Zymo Quick 16S NGS library preparation kit (Zymo Research). The final libraries were pooled and sequenced with MiSeq Sequencing System (Illumina) for 2x250 base paired-end reads.

Submitted by: University of Genoa

Study: Mobi-DEE_1 Pediatric epilepsy gut microbiome study

PRJNA1133091 • SRP518581 • All experiments • All runs

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Patients with epilepsy are known to have intestinal dysbiosis, which can modulate drug-response and disease outcome. We investigated the composition of the gut microbiota in a cohort of pediatric patients with epilepsy of genetic and presumed genetic etiologies. Through 16S analysis we show that epileptic patients, and in particular those who are drug-resistant, display unique gut metagenomic signatures compared to age-matched neurotypical controls. This study shows that the gut-brain axis can have profound effects on health and disease. The characterization of metagenomic profiles associated with epilepsy could lead to the discovery of novel biomarkers and therapeutic approaches for this multifaceted disease.

Sample:

SAMN42378026 • SRS21930780 • All experiments • All runs

Organism: human gut metagenome

Library:

Name: S6575Nr74

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 61,360 spots, 30.8M bases, 16.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR29743760	61,360	30.8M	16.4Mb	2025-01-01

ID:: 33586562

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